A 500 ml or 1000 ml sterile graduated cylinder may be used to collect the flushing medium. The embryos will settle to the bottom by gravity. After a 15 minute wait the upper portion of the fluid may be gently siphoned off by lowering a small diameter length of tubing to the 50 mm mark. The remaining smaller volume may then be poured into a searching dish and examined under the microscope for the presence of embryos. This method is less expensive than the use of single-use disposable embryo filters.
Drost M (1984)